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. 2023 Jul 25;14:4477. doi: 10.1038/s41467-023-40251-8

Fig. 4. Relationship between CaKAN3 and CaHSF8 in the regulation of the six tested NLRs at 1 hpt of HTHH treatment.

Fig. 4

a, b The 6 tested NLR genes, CaR1B23, CaR1B11, CaR1A, CaR1B12, CaR1A6 and CaR1B-16, were upregulated by CaHSF8 transient overexpression, but this upregulation was blocked by CaKAN3 silencing in pepper plants at 1 hpt of HTHH, and vice versa. The 6 NLR genes were downregulated by CaKAN3 transient overexpression at 0 hpt and by CaHSF8 silencing, and CaKAN3 and CaHSF8 functioned independently at 0 hpt of HTHH. c The co-transient overexpression of CaKAN3 and CaHSF8 induced higher expression levels of the 6 tested NLR genes than transient overexpression of CaKAN3 and CaHSF8 individually at 28 °C. d By ChIP‒qPCR, CaKAN3 silencing significantly reduced the enrichment of CaHSF8 on the promoters of the tested NLR genes, but CaHSF8 silencing did not reduce that of CaKAN3 on these promoters. The enrichment of IP:anti-HA was set to 1 after normalization by input. The ratio of IP:anti-HA to IP:IgG is indicated on the error line of IP:IgG. In a–c CaActin was used as an internal control. Data are shown as the means ± standard errors of four replicates. Different uppercase letters above the bars indicate significant differences (P < 0.01) by Fisher’s protected LSD test. All replicates were from different plants. In a–d, source data are provided as a Source Data file.