Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jul 25;14:4481. doi: 10.1038/s41467-023-40076-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a Box plots of IFNα protein in BAL from CC x K18-hACE2 mice at 3 dpi. b, c IFNα protein in BAL from (b) male and (c) female resistant (PWK, NZO and 129S1 x K18-hACE2) and sensitive (K18-hACE2 and A/J x K18-hACE2) mice at 3 and 6 dpi. Biological replicates were examined over at least 2 independent experiments and the numbers of mice assessed per strain (male: female) were as follows: 3 dpi (K18-hACE2 (5:7); A/J x K18-hACE2 (5:5); PWK x K18-hACE2 (7:6); NZO x K18-hACE2 (7:7); 129S1 x K18-hACE2 (6:5); CAST x K18-hACE2 (8:6); NOD x K18-hACE2 (5:4); WSB x K18-hACE2 (6:9), and 6 dpi (K18-hACE2 (6:6); A/J x K18-hACE2 (6:7); PWK x K18-hACE2 (6:7); NZO x K18-hACE2 (7:5); 129S1 x K18-hACE2 (5:5); CAST x K18-hACE2 (8:9); NOD x K18-hACE2 (4:6); WSB x K18-hACE2 (5:7). Two-tailed Student t-test with 95% confidence interval was used to compare cytokine production in (a) male versus female for each strain, (b) resistant versus sensitive males, and (c) resistant versus sensitive females. *p < 0.05 was considered statistically significant. d Heatmap of cytokine and chemokine responses in BAL. e Box plots of resolving group A cytokines in resistant CC x K18-hACE2 mice (IL-6, IL-27, CCL2, CXCL10), or increasing group B cytokines in sensitive CC x K18-hACE2 mice (IL-10, CCL4). Box plots show data from individual mice (squares, circles, diamonds), the horizontal dashed lines represent the median value, the box indicates the first and third quartiles, and the whiskers indicate the maxima and minima values (Qlucore software). Source data are provided as a Source Data file. Two-tailed, unpaired Student’s t-test with Holm-Bonferroni multiple comparison posttest and 95% confidence interval were used to compare cytokine production in resistant versus sensitive strains (males and females combined). *p<0.05 was considered statistically significant. f, g Correlation plots of IFNα and cytokine panel for K18-hACE2 and PWK x K18-hACE2 at (f) 3 dpi and (g) 6 dpi. Correlation analyses were performed by computing Spearman’s rank correlation coefficients. Correlation tests with p < 0.001 were displayed.