Fig. 1. Canonical pathways of genes differentially expressed in the spleen following LPS injection.

A Experimental protocol. Adult mice were i.p. injected with LPS (1.0 mg/kg) 6 days after i.p. administration of arketamine (10 mg/kg) or saline (10 ml/kg). B For RNA-sequencing, spleen samples were collected 24 h after injection of LPS. C Top five canonical pathways altered in the spleen were identified by Ingenuity Pathway Analysis (IPA). The heme biosynthesis II signaling pathway had the highest inhibition scores (P = 1.02E-06, z-score = −2.00). IPA z-score indicates whether the pathway is predicated to be inhibited (blue) or activated (red). In some cases, activation or inhibition cannot be predicted (gray).