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. 2023 Jul 25;6:775. doi: 10.1038/s42003-023-05143-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a LC-MS analysis showed that the content of the NAD metabolic pathway decreased significantly after NFIB knockdown in HT29 cells. b, c The NAD/NADH level of HT29 and SW480 cells decreased significantly after knocking down NFIB. The CCK-8 assays of HT29 (d) or SW480 (e) cells were treated with NMN (100 μM) for 3 days. f, g The NAD+ levels of HT29 or SW480 cells were treated with NMN (100 μM) for 3 days. Bar graph data are presented as the mean ± SD; *P < 0.05; **P < 0.01; ***P < 0.001.