Figure 5.

Robust induction of inflammatory cytokines by clinical Pa strain DH1137 is minimally affected by CFTR modulators. (A) CF MDM (n = 7) were treated for 48 h in vitro with CFTR modulators, then media was exchanged and a subset were infected with DH1137 at an MOI of 10 for 2 h. Supernatants were spun down and collected. Cytokine multiplex was performed on aggregated samples with 2–4 technical replicates per point. Means for each subject were log-transformed and graphed as indicated. (B) nonCF MDM (n = 5) were used in otherwise identical experiments to (A).