Fig. 1. CircMET is a potential coding circRNA subjected to m⁶A modification.

a Experimental strategy. GSC, glioma stem cell. NSC, neural stem cell. DEcircRNAs, differentially expressed circRNAs. b Circos plot shows the genome-wide m⁶A-modified circRNAs in GBM tissues. The outer circle represents the karyotype and centromere of chromosomes. The height of blue and red ticks represents the number of circRNAs detected (1~10) in input RNA-seq or m⁶A-seq, respectively. The Venn diagram in the centre illustrates the exact number of circRNAs detected in the m⁶A-seq and RNA-seq input data. c Proportion of the source regions of m⁶A circRNAs and non-m⁶A circRNAs. d KEGG enrichment analysis of the source genes of m⁶A circRNAs. Hypergeometric test. The exact P values are provided in Supplementary Data 2. e Volcano plot showing differentially expressed circRNAs between GSCs and NSCs identified from the RNC-seq data. f Upset plot illustrating the number of circRNAs detected by m⁶A-seq, RNC-seq and YTHDF2 RIP-seq. g Five candidate circRNAs were screened from the overlap of the m⁶A-seq, RNC-seq and YTHDF2 RIP-seq data. The yellow and blue polygons represent the RNC-seq expression levels of circRNAs in GSCs and NSCs, respectively. Source data are provided in the supplementary datasets.