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. 2023 Jul 25;14:4467. doi: 10.1038/s41467-023-40212-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Bubble plot of the KEGG enrichment analysis results based on bulk RNA-seq data of GSCs with stable knockdown (KD) or overexpression (OE). n = 3 biologically independent samples for each group. b Protein levels of phospho-MET and downstream phospho-AKT (S473) and phospho-ERK signals in stable circMET KD cells. Representative of three independent experiments. c Proliferation of control and circMET stable KD GSC456 and GSC23 cells. n = 5 independent experiments. Two-way ANOVA test. GSC456, scr vs sh1, P = 3.0e−15; scr vs sh2, P = 1.8e−14. GSC23, scr vs sh1, P < 1.0e−15; scr vs sh2, P < 1.0e−15. d Quantification of the EdU incorporation assay of the control and stable circMET KD GSC456 and GSC23 cells. n = 5 independent experiments. GSC456, scr vs sh1, P = 2.3e−05; scr vs sh2, P = 2.4e−05. GSC23, scr vs sh1, P = 0.0081; scr vs sh2, P = 0.011. e Limited dilution assay (LDA) analysis of the control and stable circMET KD GSC456 and GSC23 cells. n = 3 independent experiments. GSC456, scr vs sh1, P = 4.78e−07; scr vs sh2, P = 1.88e−08. GSC23, scr vs sh1, P = 1.05e−06; scr vs sh2, P = 3.64e−05. f Stem cell frequencies of LDA analysis of the control and stable circMET KD GSC456 and GSC23 cells. n = 3 independent experiments.GSC456, scr vs sh1, P = 0.0176; scr vs sh2, P = 0.0136. GSC23, scr vs sh1, P = 0.0194; scr vs sh2, P = 0.0298. g Representative haematoxylin and eosin (H&E)-stained brain slices from mice intracranially injected with the control and stable circMET KD GSC456 and GSC23 cells (n = 5 per group). Scale bar, 1 mm. h Survival analysis of mice intracranially injected with the control and stable circMET KD GSC456 and GSC23 cells (n = 5 per group). Log-rank test. GSC456, scr vs sh1, P = 0.0043; scr vs sh2, P = 0.0043. GSC23, scr vs sh1, P = 0.0017; scr vs sh2, P = 0.0017. The data are presented as the mean ± SD. The statistical tests are two-sided unless otherwise specified. Unpaired Student’s t test was used to determine the significance of the differences between the indicated groups where applicable. *P < 0.05; **P < 0.01; ***P < 0.001. Source data are provided as a Source data file.