Table 1.
Results of rnaQUAST analysis for both CF and SF.
| Cavefish | Surface fish | |||
|---|---|---|---|---|
| Count | % | Count | % | |
| Transcripts | 270,293 | 100% | 244,721 | 100% |
| Transcripts > 500 bp | 130,902 | 48.43 | 111,470 | 45.55 |
| Transcripts > 1000 bp | 79,863 | 29.55 | 66,889 | 27.33 |
| Aligned | 269,974 | 99.88 | 241,147 | 98.54 |
| Uniquely aligned | 253,388 | 93.75 | 223,275 | 91.24 |
| Multiply aligned | 3,453 | 1.28 | 2,357 | 0.96 |
| Unaligned | 319 | 0.12 | 3,574 | 1.46 |
| Misassemblies | 13,133 | 4.86 | 15,515 | 6.34 |
| Avg. aligned fraction | 0.974 | 0.964 | ||
| Avg. alignment length | 980.701 | 934.444 | ||
| Avg. mismatches per transcript | 4.905 | 4.754 | ||
Most assembled transcripts were longer than 500 bp. In CF, 99.88% of transcripts were successfully aligned against the reference genome. Similarly, in SF 98.54% were aligned. Uniquely alignments represent more than 90% of the aligned transcripts in both morphotypes.