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. 2023 Jun 30;9(7):e17849. doi: 10.1016/j.heliyon.2023.e17849

Fig. 4.

Fig. 4

CircHMGA2 knockdown repressed the ferroptosis and pyroptosis in H/R-induced HCMs through downregulating NLRP3 expression. (A) The efficiency of pcDNA-mediated NLRP3 overexpression (pcDNA-NLRP3) in H/R-induced HCMs was detected by RT-qPCR. **p < 0.01 vs. vector. (B) Effect of circHMGA2 knockdown on the expression of pyroptosis-related proteins (NLRP3, cleaved caspase-1, IL-1β and GSDMD-N) was detected by western blotting in H/R-induced HCMs transfected with pcDNA-NLRP3. **p < 0.01 vs. H/R + sh-NC; #p < 0.05 and ##p < 0.01 vs. H/R + sh-circHMGA2. (C) Effect of circHMGA2 knockdown on the proliferative activity was detected by CCK-8 assay in H/R-induced HCMs transfected with pcDNA-NLRP3. *p < 0.05 vs. H/R + sh-NC; #p < 0.05 vs. H/R + sh-circHMGA2. (D) Effect of circHMGA2 knockdown on the ferrous ion (Fe2+) content in H/R-induced HCMs transfected with pcDNA-NLRP3 was detected by iron assay kit. **p < 0.01 vs. H/R + sh-NC; #p < 0.05 vs. H/R + sh-circHMGA2. (E–F) Effect of circHMGA2 knockdown on ROS levels in H/R-induced HCMs transfected with pcDNA-NLRP3 was detected by DCFH-DA staining. The staining was shown in (E) and quantifications were as described in (F). **p < 0.01 vs. H/R + sh-NC; #p < 0.05 vs. H/R + sh-circHMGA2. (G) Effect of circHMGA2 knockdown on the expression of inflammatory factors, including IL-1β and IL-10 was detected by ELISA assays in H/R-induced HCMs transfected with pcDNA-NLRP3. *p < 0.05 vs. H/R + sh-NC; #p < 0.05 vs. H/R + sh-circHMGA2.