FIG. 2.

Genetic organization of the region between the cutE (lnt) gene and the nag operon at 14.8 to 15.1 min of the E. coli chromosome. Previously identified genes and their directions of transcription are indicated by solid arrows. The tRNA cluster is represented by a solid box. Shaded arrows indicate putative ORFs. (There is also an alternative nomenclature for the E. coli ORFs, where f474 is denoted yleA, o391 is yleB, f359a is ybeZ, and f155b is ybeY.) The vertical arrow indicates the point where the miaB::Tn10dCm transposon had been inserted. Horizontal bars represent the inserts of the S. typhimurium plasmids. Plasmids pUST138 and pUST190 are high-copy number and low-copy number versions, respectively, carrying the same insert.