FIG. 5.

Mapping of the 5′ ends of the miaB (A) and the o391 (B) mRNA by primer extension analyses with RNA isolated from S. typhimurium grown at 37°C. The Sekv14 primer was used in both sequencing and extension reactions for mapping of the miaB transcripts, while the PCR2 primer was used in mapping of the o391 transcriptional start point. Lanes A, C, G, and T represent the DNA-sequencing ladder. Lanes: 1, pUST138 plasmid in GT522 background; 2, pUST137 plasmid in GT522 background; 3, GT522 without any plasmid.