TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| E. coli | ||
| DH5α | supE44 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 ΔlacU169 (φ80 lacZΔM15) | Bethesda Research Laboratories |
| CC104 | F′ lacI378 lacZ-GCG proB+/ara Δ(lac-pro)XIII | 14 |
| TX3325 | mutS::Tn10 miaB::Tn10dCm | This work |
| TX3346 | F′ lacI378 lacZ-GCG proB+/ara Δ(lac-pro)XIIImiaB::Tn10dCm | This work |
| S. typhimurium | ||
| GT522 | Wild type | 19 |
| GT907 | metA22 metE551 ilv-452 trpB2 xyl-404 rpsL120 flaA66 hsdL66 hsdA29 galE503 | This work |
| GT1825 | hisO1242 hisD6404(Am) leuA414(Am) supF30 pro-688::Tn5/F′ del-14 lacIam 117 proAB+ | 20 |
| GT1975 | hisO1242 hisD6404(Am) leuA414(Am) supF30 pro-688::Tn5 miaB2508::Tn10dCm/F′ del-14 lacIam 117 proAB+ | 20 |
| GT2176a | miaB2508::Tn10dCm phs | 20 |
| GT2734a | miaB2508::Tn10dCm | This work |
| GT3171 | miaB2508::Tn10dCm zbf-99::Tn10 | This work |
| GT3828 | miaB26 hisO1242 hisD6404(Am) leuA414(Am) supF30 pro-688::Tn5/F′ del-14 lacIam117 proAB+ | This work |
| GT3830 | miaB27 hisO1242 hisD6404(Am) leuA414(Am) supF30 pro-688::Tn5/F′ del-14 lacIam117 proAB+ | This work |
| GT3831 | miaB28 hisO1242 hisD6404(Am) leuA414(Am) supF30 pro-688::Tn5/F′ del-14 lacIam117 proAB+ | This work |
| GT3833 | miaB29 hisO1242 hisD6404(Am) leuA414(Am) supF30 pro-688::Tn5/F′ del-14 lacIam117 proAB+ | This work |
| GT5441 | pUST138/miaB2508::Tn10dCm | This work |
| GT5442 | pBluescript SK/miaB2508::Tn10dCm | This work |
| GT5443 | zbf-99::Tn10 miaB26 | This work |
| GT5444 | zbf-99::Tn10 miaB27 | This work |
| GT5445 | zbf-99::Tn10 miaB28 | This work |
| GT5446 | zbf-99::Tn10 miaB29 | This work |
| GT5766 | pUST190/zbf-99::Tn10 miaB26 | This work |
| GT5767 | pUST190/zbf-99::Tn10 miaB27 | This work |
| GT5768 | pUST190/zbf-99::Tn10 miaB28 | This work |
| GT5769 | pUST190/zbf-99::Tn10 miaB29 | This work |
| MST1934 | galE496 mutL111::Tn10 metA22 metE55 rpsL120 xyl-404 fels2 HI-6 nml H2 enx hsdL-6 hsdA29 | S. R. Maloy |
| TT2342 | zbf-99::Tn10 supE(Su2) hisC527 leuA414 | J. R. Roth |
| Plasmids | ||
| pTX637 | pGEM3Z containing a 629-bp PCR fragment amplified from strain CC104 chromosome DNA with MiaBP1 and MiaBP2 primers | This work |
| pTX638 | pGEM3Z containing a 948-bp PCR fragment amplified from strain CC104 chromosome DNA with MiaBP1 and MiaBP2 primers | This work |
| pTX639 | pGEM3Z containing a 641-bp PCR fragment amplified from strain CC104 chromosome DNA with MiaBT1 and MiaBT2 primers | This work |
| pTX640 | pGEM3Z containing a 950-bp PCR fragment amplified from strain CC104 chromosome DNA with MiaBT1 and MiaBT3 primers | This work |
| pTX641 | Apr pBR322 vector containing a 2.4-kb chromosomal AatII insert from E. coli carrying the Tn10dCm inserted in the miaB gene | This work |
| pTX642 | Apr pKK223-3 vector containing a 1.55-kb E. coli miaB+ PCR fragment generated by amplification with the miaBSmaI and miaBPstI primers | This work |
| pUST135 | Cbr pT7Blue(R) vector containing a 1.3-kb fragment generated by PCR amplification of the GT3171 chromosome with the Tn10XbaI and T-L primers | This work |
| pUST136 | Kmr pLG339 vector containing a 5.5-kb chromosomal S. typhimurium Sau3A fragment including the miaB gene | This work |
| pUST137 | Cbr pBluescript SK vector carrying a 3.7-kb miaB+ NarI-ClaI fragment from pUST136 | This work |
| pUST138 | Cbr pBluescript SK vector carrying a 2.3-kb miaB+ fragment from pUST137 | This work |
| pUST190 | Strr pCL1921 vector carrying a 2.3-kb miaB+ NarI-NruI fragment from pUST136 | This work |
a
In reference 20, the genotype of strain GT2176 was reported as miaB2508::Tn10dCm. However, we found that this strain harbored an additional mutation. This second mutation, possibly located in the phs locus, rendered cells unable to produce H2S. This phenotype was not linked to the miaB mutation, and a new miaB2508::Tn10dCm strain (GT2734) was constructed by P22 transduction.