. 1999 Dec;181(23):7256–7265. doi: 10.1128/jb.181.23.7256-7265.1999

TABLE 3.

Presence of the different i⁶A derivatives in the tRNA of S. typhimurium carrying wild-type and mutant miaB alleles

Strain	Relevant genotype^a	Plasmid	Growth temp (°C)	Ratio of i⁶A derivative^b to m²A
Strain	Relevant genotype^a	Plasmid	Growth temp (°C)	i⁶A	io⁶A	ms²i⁶A	ms²io⁶A
GT2734	miaB2508::Tn10dCm		37	0.55	0.068	—	—
GT5441	miaB2508::Tn10dCm	pUST138	37	0.008	0.006	0.12	0.48
GT5442	miaB2508::Tn10dCm	pBluescript SK	37	0.45	0.057	—	—
GT1825	miaB⁺		37	0.007	0.007	0.19	0.48
			41	0.012	0.029	—	0.83
GT3828	miaB26		37	0.060	0.007	0.21	0.37
			41	0.36	0.008	—	0.075
GT3830	miaB27		37	0.45	—	—	—
			41	0.42	—	—	—
GT3831	miaB28		37	0.44	—	—	—
			41	0.43	0.010	—	—
GT3833	miaB29		37	0.43	0.013	—	—
			41	0.41	—	—	—
TT2342	miaB⁺ zbf-99::Tn10		41	0.006	0.010	0.14	0.54
GT5443	miaB26 zbf-99::Tn10		41	0.36	0.008	0.070	0.077
GT5444	miaB27 zbf-99::Tn10		41	0.39	0.023	—	—
GT5445	miaB28 zbf-99::Tn10		41	0.37	0.025	—	—
GT5446	miaB29 zbf-99::Tn10		41	0.44	0.018	—	—
GT5766	miaB26 zbf-99::Tn10	pUST190	41	—	—	0.21	0.44
GT5767	miaB27 zbf-99::Tn10	pUST190	41	—	—	0.21	0.46
GT5768	miaB28 zbf-99::Tn10	pUST190	41	—	—	0.23	0.44
GT5769	miaB29 zbf-99::Tn10	pUST190	41	—	—	0.19	0.45

See Table 1 for complete genotypes.

The i⁶A derivative present in the largest amount in each strain is shown in bold letters. —, not detected. Compounds present at amounts lower than 0.005 (less than 1% of the total amount of i⁶A-derivatives) cannot be detected.