Table 1.
List of papers employing mass spectrometry based metabolomics approaches to describe the metabolic state of TAMs populations.
| Study | Specimens | TAMs populations | Metabolic pathways | Trend of metabolic alterations |
|---|---|---|---|---|
| (74) | Subcutaneous colon adenocarcinoma mouse model | General macrophages (CD11b+ sorted cells) | Glycolysis TCA cycle Glutamine Methionine metabolism |
Increased in CD11b+ resident tumor cells relative to the spleen resident cells) |
| (75) | Orthotropic mouse model of ovarian cancer | General macrophages (F4/80+ sorted cells). | Glycolysis Polyamines TCA cycle |
Increased in resident peritoneal macrophages Mϕ of bearing tumor mice relative to peritoneal resident naive Mϕ |
| (76) | Human pancreatic ductal adenocarcinoma (PDAC) cell lines | Mixed M1, M2 phenotype (in-vitro conditioned monocytes CD68+, CD163+, M-CSFR, and CD206+) | Glycolysis | Increased in conditioned TAMs relative to control monocytes |
| (77) | Orthotropic mouse model of PDAC | General macrophages (CD45+ sorted cells) | Glycolysis | Increased in resident tumor CD45+ TAMs relative to the non-tumor bearing controls cells |
| (78) | Subcutaneous Lewis Lung carcinoma mouse model | M1-like and M2-like TAMs (CD11b+Ly6G−Ly6ClowMHC-IIlow and CD11b+Ly6G−Ly6ClowMHC-IIhigh sorted cells) | TCA cycle Glutamine metabolism |
Increased in tumor resident MHC-IIhi TAMs relative to the Ly6ClowMHC-IIlow |
| (72) | Human PDAC cell lines | M2 macrophages (in-vitro conditioned murine bone-marrow-monocytes) | Pyrimidines | Increased in conditioned murine M2 like TAMs relative to control macrophages |
| (79) | Non-small cell lung cancer tumor slices | M1-like TAMs (iNOS and CD68+ stained cells) | Glycolysis TCA cycle |
Increased in iNOS and CD68+ TAMs |
| (81) | Orthotropic Lewis lung mouse model | Mixed macrophages populations (SigF+/CD11c+/F480+/CD11b (MacA), F480+/CD11b+/Ly6G-/SigF-, (MacB) sorted cells) | Eicosanoids | Increased in MacA relative to MacB populations |
| (73) | Gastric cancer cell line | M2-like TAMs (in-vitro conditioned CD206, CD163, TGFβ, Arg‐1 murine bone-marrow cells) | Triacylglycerol | Increased in conditioned TAMs relative to control |
| (82) | High-grade serous adenocarcinoma | General macrophages (EpCAM+ CD14+ CD163+ CD206+ sorted cells) | Lysophosphatidic acid | Increased in TAMs populations |
For each paper, the reference, the specimens, the TAMs populations selected in the study, the altered metabolic pathway and the trend of deregulation.