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. 2023 Jul 12;14:1213285. doi: 10.3389/fimmu.2023.1213285

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Copyright © 2023 Zhou, Yuzhakov, Behloul, Wang, Bhagat, Chu, Zhang, Cheng, Fan, Huang and Mirabella

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Schematic workflow for THP-1 cell differentiation, followed by the coculture of differentiated THP-1 cells with E7 (11-19) antigen specific T cells (ASTCs). DC: dendritic cells (putative) obtained from differentiated THP-1 cells. (B) Morphology changes THP-1 cells undergo while differentiating from naïve to immature and mature dendritic cells (iDC, mDC). (C) ELISpot assay quantifying the IFNγ positive spot forming units (SFU) derived from the E7(11-19) ASTCs reacting to antigen presentation by THP-1-derived DCs transfected with different concentrations of mRNA.