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Serial dilutions (2.0 × 104, 2.0 × 103, 2.0 × 102, 2.0 × 101, 2.0 × 100, and 2.0 × 10−1 copies) of HIV-1 plasmids were used as templates, and DW was used as the blank control. Results were simultaneously analyzed through visual reagent L-HNB (A) and the AuNPs-LFA (B). A sensitivity analysis of the HIV-1-LAMP assay indicated its LoD was 20 copies per test. DW, distilled water; CL, control line; TL, test line.
