TABLE 1.

The HIV-1-RT-LAMP-AuNPs-LFA primers used in this study.

Primer name	Sequence and modifications	Length	Gene
F3	5′-ATGGCAGTATTCAT(T/C)CACAAT-3′	21 nt	pol
B3	5′-CTACTGCCCCTTCACCT-3′	17 nt
FIP	5′-GTATGTCTGTTGCTATTAT(G/A)TCTA(T/C)-TAAAAGAAAAGGGGGGATTGG-3′	46 mer
FIP*	5′-FAM-GTATGTCTGTTGCTATTAT(G/A)TCTA(T/C)-TAAAAGAAAAGGGGGGATTGG-3′	46 mer
BIP	5′-TCAAAATTTTCGGGTTTATTACAG(G/A)- AG(T/G)AG(T/C)TT(T/)GCTGGTCCTT-3′	44 mer
LF	5′-TCTTTCCCCTGCACTGTAC-3′	19 nt
LF*	5′-Biotin-TCTTTCCCCTGCACTGTAC-3′	19 nt
LB	5′-ACAGCAGAGA(C/T)CC(A/C)(A/C)(T/G)TTG-3′	19 nt

pol-FIP*, 5′-labeled with FAM, pol-LF*, 5′-labeled with biotin, when used for the AuNPs-LFA assay. FAM, 6-carboxy-fluorescein; nt, nucleotide; mer, monomeric unit.