TABLE 4.

Comparisons of the commonly used RT-LAMP-based methods for detection of HIV-1.

Results detection technique	Advantages	Disadvantages	References
Agarose gel electrophoresis	High specificity and sensitivity	Require special equipment for agarose gel electrophoresis; time-consuming; need open the reaction tube for results identification	Curtis et al., 2012 Current study
Turbidity	High specificity; without opening the reaction tubes for results identification	Require special equipment for turbidity measurement	Hosaka et al., 2009 Current study
Fluorescence probe	High specificity and sensitivity; without opening the reaction tubes for results identification	Require expensive equipment for fluorescence signal detection	Ocwieja et al., 2015; Zhang et al., 2022
Visual reagent	High specificity; without opening the reaction tubes for results identification	The results are ambiguous when the LAMP product concentrations are low	Khan et al., 2023 Current study
AuNPs-LFA	High sensitivity and specificity; accurate and visual interpretation; easy to operate and cost-saving	Need open the reaction tube for results identification	Current study