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Changes in uhpT-lacZ expression in response to the presence of CAP and variations in RpoA and RpoD. Host strains were crp+ (A and C) and Δcrp (B and D). (A and B) Plasmids encoding wild-type RpoD (open bars), RpoD-E570A (shaded bars), and RpoD-D575A (cross-hatched bars). (C and D) Plasmids encoding wild-type RpoA (open bars) and RpoA-Δ256 (filled bars). Values are β-galactosidase activities in Miller units. Error bars indicate standard deviations.
