FIG. 3.

Transcription of the uhpT and lacUV5 promoters by wild-type (WT) RNAP and mutant RNAPs carrying RpoD variants E570A, D575A, E591A, K593A, L598A, and R599A. Reactions were carried out in the absence and presence of 20 nM CAP, as indicated. (A) The ratios of the amounts of the uhpT and the lac transcripts were determined by PhosphorImager quantitation. Transcription of uhpT was measured in the presence of UhpA. (B) uhpT transcription (Txn) in the presence of UhpA without and with CAP as indicated, calculated relative to the amount of transcription produced by the wild-type RNAP in the presence of UhpA.