TABLE 1.
Bacterial strains and plasmids used in this work
| Strain or plasmid | Relevant characteristics | Reference(s) or source |
|---|---|---|
| Strains | ||
| MC4100 | ΔlacU169 araD139 deoC1 flb5301 ptsF25 rbsR rpsL150 | 37 |
| RK1309 | MC4100 λRZ5(Km) uhpT-lacZ | 27 |
| XE65.2 | Δcrp39 ΔlacX74 rpsL thi | R. Ebright |
| TM37 | XE65.2::λRZ5(Km) uhpT-lacZ | T. Merkel |
| CAG20154 | galK thi-1 thr-1 leu-6 lacY1 tonA21 supE44 Ω(Cm) Ptrp-rpoD | 25 |
| IO697 | TM37 Ω(Cm) Ptrp-rpoD | P1(CAG20154) × TM37 |
| Plasmids | ||
| pRJK10 | ApruhpABCT | 43 |
| pLAW2 | AprrpoA | 47 |
| pLAD256 | AprrpoA-256 (encoding RNAP α subunit deleted at codon 256) | 16 |
| pHTf1α (and derivatives) | AprrpoA (derivatives carrying αCTD alanine substitutions at positions 255 to 271) | 12, 41 |
| pREIIα (and derivatives) | AprrpoA (derivatives carrying αCTD alanine substitutions at positions 273 to 329) | 3, 12, 34 |
| pHTT7f1NHα | AprrpoA (derivatives carrying αCTD alanine substitutions with His6-coding region, T7 promoter) | 12 |
| pGEX-2Tς70 (and derivatives) | AprrpoD (derivatives carrying RNAP ς70 subunit alanine substitutions at positions 574, 590 to 613) | 9, 25 |
| pVRς (and derivatives) | AprrpoD (derivatives carrying RNAP ς70 subunit alanine substitutions at positions 570 and 575) | 23 |
| pGEM-His6 (and derivatives) | AprrpoD (derivatives carrying His6-RNAP ς70 subunit alanine substitutions, T7 promoter) | P. Landini |
| pMKSe2 | AprrpoB, lacP control | R. Gourse |
| pT7β′ | AprrpoC, T7 promoter control | 45 |
| pLHN12s | AprrpoD, T7 promoter control | 34 |