Figure 3.
A subset of cancer cells demonstrates irregular responses to acetylcholine stimulation independently of their specific pattern of muscarinic or nicotinic receptor expression
(A) Representative examples of Ca2+ traces of “irregular responders” – cancer cell lines showing some responses to Ach that do not correlate with the onset of agonist addition.
(B) Clustering of the cell lines used in the study, quantified by proportion of individual cells behaving as regular, irregular, or no-responders.
(C–D) Expression levels of muscarinic M3 receptor (C) or other Ach receptor (D) genes across the cell lines. No correlation between the response type and receptor expression pattern can be seen. LS174T cells are not included in the analysis as they are not part of the CCLE database.