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. 2023 Jul 11;65:102816. doi: 10.1016/j.redox.2023.102816

Fig. 1.

Fig. 1

Antioxidant effect of MitoQ in DHT-stimulated RWPE-1 cells. (A) Molecular structure of MitoQ. (B) Viability of MitoQ-treated RWPE-1 cells at the indicated concentration for 24 h measured via the CCK-8 assay. (C) RWPE-1 cell proliferation under DHT (10 nM) stimulation and MitoQ at the indicated concentration for 24 h measured via the CCK-8 assay. (D) The expression of 8OHDG in DHT-stimulated RWPE-1 cells treated with MitoQ (25, 50, and 100 μM). (E) The mRNA expression of HO-1 and GPX-1 in DHT-stimulated RWPE-1 cells treated with MitoQ (25, 50, and 100 μM). The results are expressed as means ± SD (n = 3). ###p < 0.001 vs. vehicle group; **p < 0.01, ***p < 0.001 vs. DHT-stimulated group. (F) The manifestation of MitoTracker™ and MitoSOX™ in DHT-stimulated MitoQ-treated RWPE-1 cells (100 μM); 0.6% H2O2 was used as positive control. (G) Catalase and SOD2 protein expression in DHT-stimulated RWPE-1 cells treated with MitoQ (25, 50, and 100 μM). The results are expressed as means ± SD (n = 3). ###p < 0.001 vs. vehicle group; **p < 0.01, ***p < 0.001 vs. DHT-stimulated group.