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. 2023 Jul 11;65:102816. doi: 10.1016/j.redox.2023.102816

Fig. 2.

Fig. 2

Effect of MitoQ on the NLRP3 signaling pathway in DHT-stimulated RWPE-1 cells. (A) NLRP3 expression in DHT-stimulated RWPE-1 cells treated with MitoQ (25, 50, and 100 μM). (B) The mRNA expression of NLRP3, PYCARD, and IL-1β in DHT-stimulated RWPE-1 cells treated with MitoQ (25, 50, and 100 μM). The results are expressed as means ± SD (n = 3). ###p < 0.001 vs. vehicle group; *p < 0.05, **p < 0.01, ***p < 0.001 vs. DHT-stimulated group. (C) RWPE-1 cells were transfected with GFP or NLRP3 siRNA and stimulated with or without DHT, and then transfected with NLRP3 siRNA. The mRNA expression of NLRP3 and IL-1β was estimated in transfected RWPE-1 cells. ###p < 0.001 vs. GFP group; ***p < 0.001 vs. DHT-stimulated GFP group; $$$p < 0.001 vs. DHT-stimulated NLRP3 siRNA-transfected group. (D) Molecular docking simulation on the NACHT domain of NLRP3 was conducted using Autodock Vina v1.1.2. (E) The ATPase activity in MitoQ-treated RWPE-1 cells was analyzed under DHT stimulation. (F) ATP level in DHT-stimulated RWPE-1 cells treated with MitoQ (25, 50, and 100 μM). The results are expressed as means ± SD (n = 3). ###p < 0.001 vs. vehicle group; ***p < 0.001 vs. DHT-stimulated group.