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. 2023 Jul 11;65:102816. doi: 10.1016/j.redox.2023.102816

Fig. 3.

Fig. 3

Inhibitory effect of MitoQ on AR-dependent cellular proliferation in DHT-stimulated RWPE-1 cells. (A) AR protein expression in DHT-stimulated RWPE-1 cells treated with MitoQ (25, 50, and 100 μM). (B) The mRNA levels of AR, 5α-reductase, and PSA in DHT-stimulated RWPE-1 cells treated with MitoQ (25, 50, and 100 μM). (C) PSA protein level in DHT-stimulated RWPE-1 cells treated with MitoQ (25, 50, and 100 μM). (D) Under DHT stimulation, mRNA expression of AR in NLRP3 siRNA-transfected RWPE-1 cells with or without 100 μM of MitoQ and (E) the mRNA expression of NLRP3 in AR siRNA-transfected RWPE-1 cells with or without 100 μM of MitoQ. The results are expressed as means ± SD (n = 3). ###p < 0.001 vs. GFP group; ***p < 0.001 vs. DHT-stimulated GFP group; $$$p < 0.001 vs. DHT-stimulated NLRP3 siRNA or AR siRNA-transfected group. (F) Molecular docking simulation of AR was conducted using Autodock Vina v1.1.2.