Fig. 4.

Effect of MitoQ on prostate cell proliferation in rats with BPH. (A) Representative prostate pictures from each experimental group. (B) Prostate weight to body weight (PW/BW) ratio was calculated at the end of the experiments. (C) H&E and IHC staining for PCNA were conducted using prostate tissue sections. Images from each group were observed using a Leica microscope (original magnification = ×40). (D) The thickness of the epithelium of the prostate tissue was measured using Leica Application Suite software. (E) The extent of PCNA-positive units was measured based on IHC staining. (F) PCNA mRNA level in the prostate of each experimental group was quantified using qRT-PCR. ^###p < 0.001 compared with the Con group; ***p < 0.001 compared with the BPH group; analysis of variance, followed by Dunnett's post-hoc test.