Fig. 5.

Effect of MitoQ on oxidative stress in rats with BPH. (A) IF assay was conducted to detect the protein expression of 8-OHdG. (B) The extent of 8-OHdG positive unit was measured, based on IF assay. (C) ROS scavenging effect of MitoQ was measured using ROS assay. The extent of ROS positive cells from prostate was calculated. (D) The mRNA expression of HO-1, SOD1, and PGC1α was evaluated using qRT-PCR. ^###P < 0.001 compared with the Con group; ***P < 0.001 compared with the BPH group; analysis of variance, followed by Dunnett's post-hoc test. (F) The protein expression of Catalase and SOD2 in rats with BPH. The results are expressed as the mean ± SD (n = 3). ^###p < 0.001 vs. vehicle group; ***p < 0.001 compared with the BPH group.