Fig. 4.

Temporal kinome dynamics following FGF treatment. MCF-7 cells were treated with either FGF2, FGF3, FGF4, FGF10, or FGF19, together with heparin. The resulting temporal kinome dynamics were quantified using the targeted activation loop assay and normalized to the t = 0 time point. The presented kinome signaling dynamics are separated per FGF used for stimulation, and kinases are ordered based on significant initial activation (ANOVA + Tukey’s range test, p < 0.05, biological triplicates). Black and red arrows indicate whether measured kinase activity increased or decreased over time, respectively. The fold change compared to the t = 0 time point is represented by the thickness of the arrows. MARK1/2/3 phosphorylated site T215/T208/T211 is ambiguous to T219/T212/T215. PKAa/b/g phosphorylation site T196/T198/T198 is ambiguous to T200/T202/T202. FGF, fibroblast growth factor.