Figure 1.

Ube4A deletion does not alter body weight but causes mild insulin resistance in young, chow-fed mice.

A. CRISPR/Cas9-mediated targeting of the U-Box and part of the 3′-UTR of the Ube4A locus in the mouse chromosome 9.

B. Expression levels of the Ube4A protein in tissues of young, chow-fed, WT and UKO mice. Figure represents data from N = 3 mice.

C. Body weight of young, chow-fed male mice (n = 13/cohort).

D. Body composition of young, chow-fed male mice (n = 6/cohort).

E. Raw values of the GTT in young, chow-fed male mice (n = 6/cohort).

F. Area under curve (AUC) values of the GTT (% initial) (n = 6/cohort).

G. Serum insulin levels in 5 h-fasted, young, chow-fed male mice (N = 6/cohort).

H. Body weight of young, chow-fed female mice (n = 8/cohort).

I. Body composition (fat, lean and fluid mass) of young, chow-fed female mice (n = 5/cohort).

J. Raw values of the GTT in young, chow-fed female mice (n = 6–10/cohort).

K. AUC values of the GTT (% initial) in young, chow-fed female mice (n = 6–10/cohort).

L. Serum insulin levels in 5 h-fasted young, chow-fed female mice (N = 6/cohort).