Figure 3.

Ube4A deletion exacerbates HFD-induced hepatic steatosis and liver injury in male mice with increased fatty acid uptake, lipogenesis, and lipid droplet stabilization.

A. Weight of liver in HFD-fed male mice (N = 8/cohort).

B. Representative image of liver of HFD-fed male mice.

C. Liver triglyceride (TAG) levels in HFD-fed male mice (N = 4/cohort).

D. Representative images of liver histology in HFD-fed male mice. Images were taken at 4X (left panel, scale bar: 250 μm) and 40X (right panel, scale bar: 25 μm) magnifications.

E. Average values of NAFLD feature scores in HFD-fed male mouse livers (N = 5/cohort).

F. Volcano plot from the lipidomics study in HFD-fed male mouse livers (N = 5/cohort).

G. Species of TAG and DAG in HFD-fed male WT and UKO livers (N = 5/cohort).

H. Serum levels of AST and ALT in HFD-fed male mice (N = 8/cohort).

I–N. Relative mRNA expression of lipogenic genes and transcription factors in HFD-fed male mice (N = 6/cohort).

O. Uptake of [³H]-oleate in hepatocytes under basal conditions (N = 4, experimental replicates from N = 3 male mice/cohort).

P. Incorporation of [³H]-palmitate into TAG in hepatocytes (N = 12 experimental replicates from N = 4 male mice/cohort).

Q-R. Inflammatory and fibrogenic gene expression in HFD-fed male mice (N = 6/cohort).

S-T. Representative images (20X, scale bar: 100 μM) of Sirius-Red staining in HFD-fed mouse livers. For quantification, the mean value of WT was set as 1 (n = 5/cohort).

The number of mice (n) are presented as individual datapoints. Mean ± s.e.m. shown within dot plots. For multiple comparisons, two-way ANOVA with Holm-Šidák multiple comparison test and for two independent data sets, Two-tailed unpaired Student's t-test. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.