Figure 5.

In female UKO mice, HFD feeding aggravates obesity, insulin resistance, hepatic steatosis, and liver injury.

A. Weekly body weight in female mice (n = 8/cohort).

B. Representative image of HFD-fed mice.

C. Percent body composition of mice after 16 weeks of HFD-feeding (n = 6/cohort).

D. Serum insulin levels in 5 h-fasted mice after 16 weeks of HFD-feeding (n = 6/cohort).

E. Raw values of the GTT in HFD-fed mice (n = 8/cohort). GTT was performed in 12-week-HFD-fed mice.

F. AUC values of the GTT (% initial) in HFD-fed mice (n = 8/cohort).

G. Rate (percent of 0 time point) of decrease in blood glucose levels in an ITT in HFD-fed mice (n = 6/cohort). ITT was performed in 14-week-HFD-fed mice.

H. AUC values of the ITT presented in Figure 5G (n = 6/cohort).

I–K. Serum levels of TAG, NEFA and total cholesterol and in HFD-fed mice (n = 5/cohort).

L. Weight of liver in HFD-fed mice (n = 6/cohort).

M. Representative images of liver histology from HFD-fed mice. Images were taken at 4X (left panel, scale bar: 250 μm) and 40X (right panel, scale bar: 25 μm) magnifications.

N. Average values of NAFLD feature scores in HFD-fed mouse livers (N = 5/cohort).

O. Species of TAG in HFD-fed mouse livers (N = 5/cohort).

P-Q. Representative images (20X, scale bar: 100 μM) of Sirius-Red staining in HFD-fed mouse livers. For quantification, the mean value of WT was set as 1 (n = 5/cohort).

R. Serum levels of AST and ALT in HFD-fed mice (N = 8/cohort).

The number of mice (n) used are presented as individual datapoints. Mean ± s.e.m. shown within dot plots. For multiple comparisons, two-way ANOVA with Holm-Šidák multiple comparison test and for two independent data sets, Two-tailed unpaired Student's t-test. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.