Fig. 4.

Trajectory analysis of OS single cells. A. RNA Velocity vectors projected onto the UMAP embedding, indicating differentiation directionality. Botom left inset shows four types of cells (osteoblastic, osteoclastic, fibroblasts and myeloid cells). Botom left inset indicates latent time. B. Heatmap showing dynamic RNA Velocity changes in osteoblasts, fibroblasts, myeloid cells and osteoclasts along latent time. C. Identified driver genes with pronounced dynamic behavior. Dashed line indicates steady states. Levels of unspliced mRNA above or below this proportion indicate increasing or decreasing expression of a gene, respectively.