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. 2023 Jul 12;14:1219194. doi: 10.3389/fendo.2023.1219194

Figure 5.

Figure 5

Effect of maternal exercise on NO bioactivity and ROS generation in mesenteric arteries from 3M male and female offspring. (A) Representative immunoblots showing phosphorylation of eNOS at Ser1177, eNOS and β-tubulin in mesenteric arteries from male and female offspring. Quantification data measured as band density of p-eNOS/total eNOS protein (n=6 animals per group). (B, C) DHE fluorescence to measure in endothelial ROS production in frozen sections of second-order mesenteric arteries from 3M male (B) and female (C) offspring. Representative mesenteric sections (×63) showing red endothelial cells with blue DAPI (arrows). Endothelium-derived ROS production, quantified in arbitrary units as area of luminal red staining/length of luminal surface. (D, E) Concentration–response curves for endothelial-dependent vasodilatation to ACh and maximum relaxation (%) in response to 10-5 M ACh in the presence of Apocynin (3×10-4 M, NADPH oxidase inhibitor) in mesenteric arteries from male (D) and female (E) offspring (n=3-5 animals per group). * P<0.05 compared with p-WKY-SED; # P<0.05 compared with p-SHR-SED.