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. 2023 Jul 12;17:1202027. doi: 10.3389/fnins.2023.1202027

Figure 4.

Figure 4

Grafted human iPSC-derived mDA neurons without CTraS method were likely to be tumors. (A) Immunofluorescence images of the aberrantly grown grafts, which was prepared without CTraS method at 8 weeks after transplantation. Bar, 3 mm. The right side of sections is indicated by R. (B) Immunofluorescence images of the graft at 8 weeks after transplantation. Bars, 50 μm. (C) Quantification of the ratio of Ki67-positive per human nuclear antigen (HNA)-positive cells. CTraS+: n = 3, CTraS-: n = 3, mean ± SEM. *p < 0.05. (D) Immunofluorescence images of the graft at 8 weeks after transplantation. (E) Quantification of the ratio of Ki67+/HNA+ cells. CTraS+: n = 13, CTraS-: n = 2, mean ± SEM. iPSCs, induced pluripotent stem cells; CTraS, chemically transitional embryoid-body-like state; HNA, human nuclear antigen.