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Strategy for single-copy transplantation of Φ(nasR-lacZ) fusion constructs into the K. oxytoca chromosome. See Materials and Methods for details. The Φ(nasR-lacZ) operon fusion constructs, derivatives of plasmid pVJS2354, span the divergent rhaB and rhaS genes. Conventional integration-excision allelic exchange (not depicted) results in transfer of the construct to the chromosome, replacing the resident rha+ locus.
