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Primer extension analysis of nasR mRNA (see Materials and Methods for details). Total RNA was isolated from K. oxytoca VJSK2507. The assigned transcription start site (+1) and part of the ςN-dependent promoter sequence (−13) are labeled. The lanes marked G, A, T, and C show the corresponding dideoxynucleotide chain termination sequencing reactions. The lane marked PE shows the primer extension product, indicated by an arrow.
