TABLE 6.
Effects of nitrate and nitrite on expression of a chromosomal Φ(nasR-lacZ) operon fusion
| Straina | Genotypeb | β-Galactosidase sp actc with:
|
|||
|---|---|---|---|---|---|
| Gln | NO3−, Gln | NO2−, Gln | NH4+, Gln | ||
| VJSK2507 | nas+ | 67 | 22 | 28 | 7 |
| VJSK2517 | nasR131::Tn10d(Cm) | 77 | 140 | 96 | 13 |
| VJSK2512 | Δ(nasFED)135 | 70 | 140 | 32 | 8 |
| VJSK2516 | Δ(nasCB)139 | 61 | 135 | 106 | 5 |
| VJSK2529 | nasD124::Ω-Cm | 67 | 150 | 101 | 6 |
a
Derivatives of strain VJSK2216 (Table 1).
b
All strains carry ΔrhaBS::[Φ(nasR-lacZ) {−469/+71}] (Fig. 1).
c
Determined as described in Materials and Methods and expressed in arbitary (Miller) units. Cultures were aerated in media containing the indicated nitrogen sources as described in Materials and Methods.