FIGURE 2.

Dynamic monitoring the effect of HDQ on mESC-CMs using RTCA cardio system. (A) Experimental workflow used to examine the effect of HDQ on mouse ES cell-derived cardiomyocytes (mESC-CMs) proliferation and beating activity. (B) Representative kinetics showing change in cell index in the absence (control) or presence of increasing concentration of HDQ. (C) Representative images of changes in beating activity of cardiomyocytes at different time points treated or not (control) with different concentrations of HDQ. (D) Average of beating rate (1/minute) of mESC-CMs recorded at indicated time points before- (pre-treatment) and after treatment with different HDQ concentrations at different time points. Data are shown as mean ± SEM of 6 wells each from three independent experiments. *p < 0.05 vs control.