Figure 2.

ETBF enteric infection initiates systemic inflammation and induces inflammatory cytokine production by splenocytes in non-tumor-bearing mice. (A) Flow cytometry data show levels of CD11b⁺ myeloid cells, CD11b⁺F4/80⁺ macrophages, CD4⁺ T cells, CD8⁺ T cells, CD11c⁺ dendritic, and CD4⁺Foxp3⁺ T regulatory cells in spleens isolated from BALB/c mice sham control or infected with ETBF for 5 days. (B) Graphs show flow cytometry analysis of levels of cytokines in CD8⁺ and CD4⁺ T cells in the splenocytes isolated from sham control and ETBF-infected (5 days) mice after ex situ stimulation with ionomycin and PMA. (C, D) Flow cytometry data show the levels of CD11b⁺ myeloid cells, CD11b⁺F4/80⁺ macrophages, CD4⁺ T cells, CD8⁺ T cells, CD11c⁺ dendritic, and CD4⁺Foxp3⁺ T regulatory cells in lungs and livers isolated from sham control and ETBF-infected (5 days) mice. (E, F) Representative H&E images of lung and liver from sham control and ETBF-infected (5 days) mice. (G) 100×, oil immersion images showing neutrophils and B-cell accumulation in lungs of 5-day ETBF-infected mice. Plots show data from a single cohort of mice (n = 6 mice per group). Inflammatory cells are denoted by arrows and outlined regions. Data are representative of two independent experiments analyzed separately; p < 0.05*, p < 0.005**, p < 0.0001***, ns (nonsignificant).