Figure 3.

Tumor microenvironment remodeling in response to ETBF gut colonization. (A) Representative images of trichrome and IHC images of fibronectin, E-cadherin, N-cadherin, vimentin, and CD31 staining of intraductal 4T1 tumors developed in sham control and ETBF-infected mice. (B) Flow cytometry analyses of levels of CD4⁺ T cells, CD8⁺ T cells, CD11b⁺ myeloid cells, CD11b⁺F4/80⁺ macrophages, CD11c⁺ dendritic cells, and CD4⁺Foxp3⁺ T regulatory cells in intraductal 4T1 tumors developed in sham control mice or mice bearing ETBF infection for 1 week or 4 weeks. Y-axis represents the number of cells per milligram of tumor tissue. Data are representative of three independent experiments analyzed separately; p < 0.05*, p < 0.005**, p < 0.0001***, ns (nonsignificant).