Figure 2.

Three-dimensional FF- and D-OCT imaging of Aspergillus fumigatus growth on Sabouraud dextrose agar for 240 min following a 24 h incubation at 30°C in a humidified chamber. Each 2D image corresponds to a 50-µm-deep axial Z-projection of the same region of interest at different times (0, 120, and 240 min). Each 3D image corresponds to a 50-µm-deep axial volume reconstruction of the same region of interest at different times (0, 120, and 240 min). The scale bar represents 25 µm. The composite RGB DCI image translates each pixel movement as a color (red for high frequencies/fast movements, green for medium frequencies/intermediate movements, and blue for low frequencies/slow movements). The background noise was modified to white for the three-dimensional D-OCT reconstruction to enhance the visualization of dynamic contrasts within the conidial heads.