Figure 4.

Whole-volume FF- and D-OCT live imaging of Aspergillus fumigatus growth on Sabouraud dextrose agar for 100 min following a 24 h incubation at 30°C in a humidified chamber. The images display a 50-µm-deep axial Z-projection of the conidiophores (A) and conidial heads (B) at different times (0, 20, 40, 80, and 100 min). The scale bar represents 25 µm. The composite RGB DCI image translates each pixel movement as a color (red for high frequencies/fast movements, green for medium frequencies/intermediate movements, and blue for low frequencies/slow movements). The white arrows show a conidiophore with vacuole trafficking and evolving DCI signal in time. The red asterisks locate the formation of the conidial head.