Figure 3.

The impact of ISO and PH on the γδ T-cell levels and the vascular permeability of peripheral organs. (a, c, e) Representative flow cytometric profiles of γδ T cells (CD3⁺ γδ TCR⁺) in the brain, lung and blood of post-stroke WT mice. (b, d, f) Flow cytometry analysis of γδ T cells in the brain, lung and blood of post-stroke mice subjected to ISO or PH 24 and 72 hours after MCAO and (g–j) Determination of Evans blue extravasation in the brain, lung, jejunum and ileum in post-stroke mice. Data are presented as individual values plus means ± SDs (n = 6–8/group). Comparisons were determined by one-way ANOVA followed by post hoc Newman–Keuls multiple comparison tests. N.S., not significant, *P < 0.05, **P < 0.01. ISO, isolated housing; MCAO, middle cerebral artery occlusion; PH, pair housing; rhANP, recombinant human atrial natriuretic peptide.