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. 2023 Apr 5;43(8):1267–1284. doi: 10.1177/0271678X231167946

Figure 5.

Figure 5.

In vivo depleting effects of γδ TCR mAb on γδ T cells. (a) Treatment schedule. Mice were randomly assigned to ISO or PH for 14 days before MCAO or sham operation. Anti-γδ TCR monoclonal antibody (500 μg/mouse every other day for 3 days, starting 3 days before MCAO) was intravenously injected into ISO mice. PH mice and other ISO mice were sham-treated with normal hamster serum IgG. Serum, brain and lung tissues were collected on Day 1 or Day 3 post-MCAO for analysis. Behavioral tests were conducted on Day 1 or Day 3 post-MCAO. (b) Representative flow cytometric plots display the percentages of γδ T cells in the spleen, blood, lung and SI-LP. (b) The percentage of γδ T cells to total CD3+ T cells in the spleen, blood, lung and SI-LP. (d) Representative flow cytometric plots of photoconverted Kaede red γδ T cells in the SI-LP, brain and lung in post-stroke mice are shown and (e) Graph displaying the absolute numbers of Kaede red γδ T cells in the SI-LP, brain and lung. Data are presented as individual values plus means ± SDs (n = 3–5/group). Comparisons were determined by unpaired Student’s t test. N.S., not significant, *P < 0.05, **P < 0.01. ISO, isolated housing; MCAO, middle cerebral artery occlusion; PH, pair housing; rhANP, recombinant human atrial natriuretic peptide; SI-LP, lamina propria of small intestine.