Figure 6.

γδ T-cell depletion attenuated ISO-induced SAP exacerbation. (a, b) Infarct volumes were calculated as the percentage of infarct volume to whole brain. (c) Neurological deficit score. (d) Total beam breaks in the open field test. (e) The use of the contralateral paw in the cylinder test was determined by the percentage of left/total. (f) Cerebral MPO activity. (g) Bacterial loads in the lungs of post-stroke mice. (h) Pulmonary MPO activity. (i) Pulmonary edema assessed by measuring wet lung weight normalized per body weight and wet/dry lung ratio. (j) Representative H&E sections of lung and histological injury scores. Scale bar =100 μm. Western blotting analysis of IL-17A in the brain (k, m) and lung (l, n). Data are presented as individual values plus means ± SDs (n = 3–12/group). Comparisons were determined by one-way ANOVA followed by post hoc Newman–Keuls multiple comparison tests. N.S., not significant, *P < 0.05, **P < 0.01, ***P < 0.001. CFU, colony-forming units; ISO, isolated housing; MCAO, middle cerebral artery occlusion; MPO, myeloperoxidase; PH, pair housing; rhANP, recombinant human atrial natriuretic peptide; mAb, monoclonal antibody.