Skip to main content
. 2023 Apr 5;43(8):1267–1284. doi: 10.1177/0271678X231167946

Figure 7.

Figure 7.

The impact of SDV on γδ T-cell migration and IL-17A expression. (a) Treatment schedule. Mice were randomly assigned to receive SDV or sham operation 28 days before MCAO or sham surgery. Fourteen days later, mice were isolated or pair-housed for 14 days. Brains and lungs were collected on Day 1 or Day 3 post-MCAO for analysis. (b) Representative flow cytometric plots of photoconverted Kaede red γδ T cells in the SI-LP, brain and lung in post-stroke mice are shown. (c) Graph displaying the absolute numbers of Kaede red+ γδ T cells in the SI-LP, brain and lung. Western blotting analysis of IL-17A in the brain (d, e) and lung (f, g). Data are presented as individual values plus means ± SDs (n = 4/group). Comparisons were determined by one-way ANOVA followed by post hoc Newman–Keuls multiple comparison tests. N.S., not significant, *P < 0.05, **P < 0.01. ISO, isolated housing; MCAO, middle cerebral artery occlusion; PH, pair housing; rhANP, recombinant human atrial natriuretic peptide; SDV, subdiaphragmatic vagotomy; SI-LP, lamina propria of small intestine.