Figure 2.

Suv39h1-mediated H3K9me3 elevation protects against hemin-induced neuronal ferroptosis. (a–l) N2A cells were treated with hemin or vehicle as indicated, and the levels of different histone modifications were assessed using Western blot. α-tubulin serves as an internal control. Representative images (a, e, i, k) and quantifications (b–d, f–h, j, l) are shown. (m, n) N2A cells were treated as Continued.indicated. Total mRNA was extracted and Real-time RT-PCR was performed. GAPDH serves as an internal control. (o) N2A cells were treated as indicated. The protein level of Suv39h1 was detected using Western blot (up) and quantified (down). (p, q) N2A cells were treated as indicated, and PI staining was performed. Representative images (p) and quantifications (q) are shown. (r, s) Cell death and lipid ROS were detected. Results are shown as scatter plots (Mean ± SD). n = 3–4 independent experiments. One-way ANOVA followed by Tukey's multiple comparisons tests (b–d, f–h, j, l, m, n, q, r, s) or two-tailed t test (o) was used. *p < 0.05, **p < 0.01, ***p < 0.001 vs vehicle; ##p < 0.01, ###p < 0.001 vs. Hemin; NS, not significant. Scale bar: 100 μm.