TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Origin	Coordinates of TP901-1 fragment(s)a	Antibiotic resistance	Reference or source
Strains
L. lactis subsp.   cremoris
JM342	MG1363 Δupp tdk			23
AB112	JM342::pAB112	−104–228	Ermr	This study
AB174a	JM342::pAB174a	−98–1910, 2521–2708	Ermr	This study
E. coli DH5α	ø80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1			Lab strain
Plasmids
pCI3340	Shuttle vector for E. coli and L. lactis		Camr	10
pCI372	Shuttle vector for E. coli and L. lactis		Camr	10
pTRKL2	Shuttle vector for E. coli and L. lactis		Ermr	25
pAK80	Shuttle vector for E. coli and L. lactis		Ermr	12
pGEM-3Zf(+)	E. coli vector		Ampr	Promega
pGEM-7Zf(+)	E. coli vector		Ampr	Promega
pIC-19R	E. coli vector		Ampr	21
pACYC184	E. coli vector		Camr	New England Biolabs
pBF12	pMOSblue-T::333-bp TP901-1 attP	−104–228	Ampr, Ermr	3
pPM92	pIC-19R::TP901-1 orf4–9	2621–5476	Ampr	20
pPM115	pCI3340::TP901-1 orf2–9	1287–5476	Camr	17
pPM129	pAK80::TP901-1 orf4–7	2621–4672	Ermr	18
pJM300	pUN121::upp		Ampr, Tetr	22
pAJ37	pCI372::TP901-1 attP, orf1–2, orf4–6d	−98–4475d	Camr	This study
pAJ39	pCI372::TP901-1 attP, orf1, orf3–6e	−98–4475e	Camr	This study
pAJ41	pCI372::TP901-1 attP, orf2–6f	−98–4475f	Camr	This study
pAJ43	pCI372::TP901-1 attP, orf1–6	−98–4475	Camr	This study
pAJ95	pAK80::TP901-1 pR-pL	3142–3332	Ermr	14
pAB35	pCI372::TP901-1 attP, orf1, orf4–6	−98–1910, 2521–4475	Camr	This study
pAB112	pMOSblue-T::333-bp TP901-1 attP, upp	−104–228	Ampr, Ermr	This study
pAB174a	pGEM-7Zf(+)::TP901-1 attP, orf1,b upp	−98–1910, 2521–2708	Ampr, Ermr	This study
pAB174b	pGEM-7Zf(+)::TP901-1 attP, orf1,cupp	−98–1910, 2521–2708	Ampr, Ermr	This study
pAB204	pGEM-3ZF(+)::upp		Ampr	This study
pAB211	pIC-19R::upp		Ampr	This study
pAB221	pCI3340::TP901-1 orf2–5	1287–3900	Camr	This study
pAB223	pCI3340::TP901-1 orf4–5	2621–3900	Camr	This study
pAB227	pTRKL2::upp		Ermr	This study
pAB235	pCI372::TP901-1 attP, orf1	−98–1910, 2521–2708	Camr	This study
pAB241	pCI3340::TP901-1 orf4–7	2621–4672	Camr	This study
pAB243	pCI3340::TP901-1 orf4–5, orf7	2621–3900, 4353–4672	Camr	This study
pAB244	pCI3340::TP901-1 orf7	4353–4672	Camr	This study
pAB245	pCI3340::TP901-1 pR-pL, orf7	3142–3332, 4353–4672	Camr	This study
pLB28	pGEM-7Zf(+)::TP901-1 attP, orf2–3	−98–2708f	Ampr	6
pLB29	pGEM-7Zf(+)::TP901-1 attP, orf1–3	−98–2708	Ampr	6
pLB44	pGEM-7Zf(+)::TP901-1 attP, orf1c	−98–1910, 2521–2708	Ampr	6
pLB55	pCI372::TP901-1 attP, orf1–2d	−98–2708d	Camr	This study
pLB56	pCI372::TP901-1 attP, orf1, orf3e	−98–2708e	Camr	This study
pLB57	pCI372::TP901-1 attP, orf2–3f	−98–2708f	Camr	This study
pLB58	pCI372::TP901-1 attP, orf1–3	−98–2708	Camr	This study
pLB61	pGEM-7Zf(+)::TP901-1 orf1	25–1910	Ampr	3
pLB71	pGEM-7Zf(+)::TP901-1 attP, orf1–2d	−98–2708d	Ampr	6
pLB72	pGEM-7Zf(+)::TP901-1 attP, orf1, orf3e	−98–2708e	Ampr	6
pLB81	pACYC184::TP901-1 orf1	25–1910	Camr	This study

^aCoordinates of the TP901-1 fragment(s), according to the numbering system introduced previously (19), where first base pair in the core region is assigned position 1 and the last base pair of the core is assigned position 5. Since the sequence of the entire phage genome is not known, we have assigned the bases on the other side of the core from orf1 negative numbers. Positive coordinates thus correspond to the sequence reported under EMBL accession no. Y14232. Negative coordinates −1 to −104 correspond to bases 2727 to 2831 in the sequence reported under EMBL accession no. X85213. 

^bThe erm gene is oriented divergently from orf1. 

^cThe erm gene is oriented in the same direction as orf1. 

^dContains an amber stop mutation in orf3. 

^eContains an amber stop mutation in orf2. 

^fContains an amber stop mutation in orf1.