Figure 9.

In vivo validation of the bone regeneration and antitumor effect of the living materials in nude mouse. a) The scheme of animal experiment. The nude mouse was injected with Matrigel contained with tumor cells (Group 1), tumor cells and hADSCs (Group 2), tumor cells and H@C@D nanorods (Group 3), and tumor cells and the living materials (Group 4). b) H&E staining images of calvarial bone defect sections at day 14. The defect area is marked with a yellow dotted line. c) Masson‐trichrome staining images of calvarial bone defect sections at day 14. The defect area is marked with a yellow dotted line. d) Immunostaining of tissue slices for the osteogenic markers and fluorescent images of PKH26‐labeled tumor cells after 21 days. Cell nuclei were stained with DAPI (blue), OCN and OPN are shown in green, while tumor cells are in green.