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Map of the pVJT128 plasmid. tnp is the IS903 transposase gene from pKD368, and kan′ is the kanamycin resistance gene from Tn903 beginning with the second codon. IR indicates the 18-bp inverted-repeat sequence of IS903. The three amber stop codons in different reading frames are indicated by XXX. The boundaries of the transposable element IS903φkan are noted. The MluI and BssHII sites used to create the transposase-minus derivative, pVJT131, are shown.
